Canine hearts preserved under hypothermic pulsatile perfusion (24 hours) with an osmolarity of 340 mOsm/L had better preservation and transplantation response than hearts perfused at an osmolarity of 310 and 270 mOsm/L. The hearts perfused at 340 mOsm /L survived an average of 22.6 days after transplantation. This survival was significantly (p>0.05) better than that observed after fresh transplantation (13.4 days average). Hearts perfused at 270 mOsm /L had a significantly (p>0.05) lower survival after transplantation (5.8 days average) than the fresh allografts or the hearts perfused at 310 or 340 mOsm / L. Similar survival times were observed for the fresh transplants and those perfused at an osmolarity of 310 mOsm/L (17.8 days average). Thus, osmolarity appears to be an essential factor for hypothermic pulsatile perfusion of hearts.
Toledo-Pereya, Luis H.; Chee, Manuel; and Lillehei, Richard C.
"Effect of Perfusate Osmolarity on Hearts Preserved Under Hypothermic Pulsatile Perfusion for 24 Hours,"
Henry Ford Hospital Medical Journal
: Vol. 26
Available at: https://scholarlycommons.henryford.com/hfhmedjournal/vol26/iss1/3