Calorie restriction for ovarian cancer reduction
Rattan R, Dimitrova I, Udumula MP, Buekers TE, and Giri S. Calorie restriction for ovarian cancer reduction. Gynecologic Oncology 2020; 159:118-119.
Objective: Dietary interventions are attractive as inexpensive supportive anticancer therapies. Calorie restriction is an established tumor preventive regimen, reducing systemic inflammation and growth factor signaling, as well as improving metabolic markers in the tumor. The aim of our study was to determine the effect of calorie restriction on ovarian cancer outcome.
Method: Female B6 mice were fed either ad libitum or underwent a 30% calorie restriction. After 5 weeks, mouse epithelial ovarian cancer (EOC) ID8 cells (5 million cells) were injected intraperitoneally. Tumor growth was monitored by in situ luciferases guided imaging, followed by pathological determination of tumors at 8 weeks. Changes in growth factors/cytokines were determined by ELISA, and immune response was measured by flow cytometry analysis.
Results: The mice on calorie restriction displayed decreased EOC burden in contrast to mice fed ad libitum (P < 0.01). The mice on calorie restriction exhibited increased survival (median survival 100 days) in contrast to mice fed ad libitum (median survival 70 days, P < 0.01). The calorie-restricted mice showed a significant reduction in levels of insulin, leptin, MCP-1, VEGF, and IL-6 (P ranging from 0.5 to 0.01). In addition, calorie-restricted mice had increased frequency of T cells (CD4, CD8, and NKT cells) and decreased frequency of macrophages (P < 0.05).
Conclusion: Our study suggests that calorie restriction can suppress ovarian cancer growth and is associated with modulation of inflammatory and immune microenvironment, suggesting the promise of calorie restriction and its mimetics as supportive anticancer therapies.