Coordinated epithelial and eosinophil inflammatory pathways underpin upper respiratory tract viral infection (URI) triggered asthma exacerbation
Altman MC, Babineau D, Whalen E, Gill MA, Shao B, Liu AH, Jepson B, Gruchalla RS, O'Connor GT, Pongracic JA, Kercsmar CM, Khurana Hershey GK, Zoratti EM, Johnson CC, Teach SJ, Kattan M, Bacharier LB, Beigelman A, Sigelman SM, Gergen PJ, Wheatley LM, Presnell S, Togias A, Busse WW, and Jackson DJ. Coordinated epithelial and eosinophil inflammatory pathways underpin upper respiratory tract viral infection (URI) triggered asthma exacerbations. J Allergy Clin Immunol 2018; 141(2 Suppl S):AB110.
J Allergy Clin Immunol
RATIONALE: Upper respiratory infections (URIs) are the primary cause of asthma exacerbations in children. Understanding the molecular mechanisms by which URIs lead to exacerbations is crucial to better management and prevention. METHODS: Children with exacerbation prone asthma and blood eosinophils >150/mm3 were enrolled and nasal lavage samples collected within 72 hours of initial URI symptoms. Nasal cell differentials were determined by cytospin and nasal gene expression assessed by RNAsequencing. 145 URIs from 104 distinct individuals were analyzed; URIs leading to asthma exacerbation (n545) were compared to URIs that resolved without exacerbation (n5100). Differential gene expression was assessed by cell deconvolution and modular analysis coupled with linear mixed effects modeling. RESULTS: URIs that led to an exacerbation demonstrated differential expression of 11 gene set modules (false discovery rate
2 Suppl S