Eosinophil gene activation in the upper airway is a marker of asthma exacerbation susceptibility in children
Jackson DJ, Babineau D, Whalen E, Gill MA, Shao B, Liu AH, Jepson B, Gruchalla RS, O'Connor GT, Pongracic JA, Kercsmar CM, Khurana Hershey GK, Zoratti EM, Johnson CC, Teach SJ, Kattan M, Bacharier LB, Beigelman A, Sigelman SM, Gergen PJ, Wheatley LM, Presnell S, Togias A, Busse WW, and Altman MC. Eosinophil gene activation in the upper airway is a marker of asthma exacerbation susceptibility in children. J Allergy Clin Immunol 2018; 141(2 Suppl S):AB114.
J Allergy Clin Immunol
RATIONALE: A significant proportion of children in urban populations remain exacerbation prone despite guideline-directed care. Preventing exacerbations in these children remains a major unmet clinical need. Identification of cellular and molecular markers of exacerbation susceptibility that can determine periods of heightened exacerbation risk is important towards improving asthma management. METHODS: 94 children with exacerbation prone asthma and peripheral blood eosinophils >150 /mm3 had nasal lavage samples collected at baseline. Nasal cell differentials were determined by cytospin and nasal gene expression assessed by RNA-sequencing. Participants were monitored for upper respiratory tract infections (URIs) and asthma exacerbations. Differential gene expression was assessed by cell deconvolution and modular analysis coupled with multivariate linear modeling. RESULTS: Baseline nasal samples were compared between children who developed an asthma exacerbation associated with a URI within 2 months of collection versus children who had a URI without asthma exacerbation. Those who developed an exacerbation had 2.7 fold higher nasal eosinophil percentages (p
2 Suppl S