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  4. Development of an online citrate/Ca2+ sensing system for dialysis

Development of an online citrate/Ca2+ sensing system for dialysis

Youjun Yang
Balazs Szamosfalvi
Jerry Yee
Stanley Frinak
Eric V. Anslyn

01-21-2011

An online citrate and Ca2+ sensing system based on sequential injection analysis (SIA) is developed as a safety module for hemodialysis. Host 1 displays high affinity towards citrate, and was selected..

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An online citrate and Ca2+ sensing system based on sequential injection analysis (SIA) is developed as a safety module for hemodialysis. Host 1 displays high affinity towards citrate, and was selected for this study owing to its unique structural features. The o-aminomethylphenylboronic moiety can effectively interact with the α-hydroxycarboxylate moiety of citrate and the remaining two guanidiniums may further stabilize the complex via hydrogen bonds. Fura-2 chelates to Ca2+ with a high selectivity and affinity and was utilized in this study for Ca2+ measurements. The citrate sensing chemistry via an indicator displacement assay is orthogonal to the Ca2+ sensing chemistry, and the use of sophisticated chemometrics is not required for data analysis. The citrate and Ca2+ concentrations in dialysate samples are measured with the developed SIA system. The obtained citrate concentrations were verified via a commercially available enzymatic assay and an NMR method, respectively, while the Ca2+ concentrations were verified via atomic absorption.

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Article

https://scholarlycommons.henryford.com/context/nephrology_articles/article/1253/viewcontent

http://scholarlycommons.henryford.com/nephrology_articles/253

20959934

http://sfxhosted.exlibrisgroup.com/hfhs?sid=Entrez:PubMed&id=pmid:20959934

Analyst

136

http://sfxhosted.exlibrisgroup.com/hfhs?sid=Entrez:PubMed&id=pmid:20959934

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