Single-molecule labeling for studying trafficking of renal transporters.
Jaykumar AB, Caceres PS, Ortiz PA. Mini review: Single molecule labeling for studying trafficking of renal transporters. Am J Physiol Renal Physiol. 2018;315(5):F1243-F1249.
American journal of physiology. Renal physiology
The ability to detect and track single molecules presents the advantage of visualizing the complex behavior of transmembrane proteins with a time and space resolution that would otherwise be lost with traditional labeling and biochemical techniques. Development of new imaging probes has provided a robust method to study their trafficking and surface dynamics. This mini-review focuses on the current technology available for single-molecule labeling of transmembrane proteins, their advantages, and limitations. We also discuss the application of these techniques to the study of renal transporter trafficking in light of recent research.