Noninvasive measurement of renal blood flow by magnetic resonance imaging in rats
Romero CA, Cabral G, Knight RA, Ding G, Peterson EL, and Carretero OA. Noninvasive measurement of renal blood flow by magnetic resonance imaging in rats. Am J Physiol Renal Physiol 2018; 314(1):F99-F106.
American journal of physiology. Renal physiology
Renal blood flow (RBF) provides important information regarding renal physiology and nephropathies. Arterial spin labeling-magnetic resonance imaging (ASL-MRI) is a noninvasive method of measuring blood flow without exogenous contrast media. However, low signal-to-noise ratio and respiratory motion artifacts are challenges for RBF measurements in small animals. Our objective was to evaluate the feasibility and reproducibility of RBF measurements by ASL-MRI using respiratory-gating and navigator correction methods to reduce motion artifacts. ASL-MRI images were obtained from the kidneys of Sprague-Dawley (SD) rats on a 7-Tesla Varian MRI system with a spin-echo imaging sequence. After 4 days, the study was repeated to evaluate its reproducibility. RBF was also measured in animals under unilateral nephrectomy and in renal artery stenosis (RST) to evaluate the sensitivity in high and low RBF models, respectively. RBF was also evaluated in Dahl salt-sensitive (SS) rats and spontaneous hypertensive rats (SHR). In SD rats, the cortical RBFs (cRBF) were 305 ± 59 and 271.8 ± 39 ml·min
Medical Subject Headings
Animals; Contrast Media; Image Processing, Computer-Assisted; Kidney; Kidney Diseases; Magnetic Resonance Imaging; Male; Rats, Sprague-Dawley; Renal Artery; Renal Circulation; Spin Labels