Beta-2 transferrin is detectable for 14 days whether refrigerated or stored at room temperature
Zervos TM, Macki M, Cook B, Schultz LR, Rock JP, Craig JR. Beta-2 transferrin is detectable for 14 days whether refrigerated or stored at room temperature. Int Forum Allergy Rhinol. 2018 Sep;8(9):1052-1055.
Int Forum Allergy Rhinol
BACKGROUND: The effect of time and temperature on beta-2 transferrin stability in cerebrospinal fluid (CSF) is not well established. After collecting nasal CSF for testing, beta-2 transferrin has been found to be stable and detectable for 1 week, whether being refrigerated or stored at room temperature. The purpose of this study was to determine if beta-2 transferrin remained detectable longer than 1 week and whether refrigeration improved its detectability.
METHODS: In patients undergoing therapeutic CSF diversion, 2-mL CSF samples were collected from 18 patients. The samples were divided and stored either at room temperature, or at 4°C, and tested for beta-2 transferrin at 7 and 14 days. CSF was collected from external ventricular drains (EVDs) (n = 15), lumbar drains (n = 2), and subdural drains (n = 1).
RESULTS: Of the 18 CSF samples originally testing positive for beta-2 transferrin, none turned negative at 7 or 14 days, in both the refrigerated and room temperature groups (95% confidence interval [CI], 0% to 18.5%).
CONCLUSION: Beta-2 transferrin remained detectable for 14 days in all CSF samples, regardless of being stored at 4°C or room temperature.
Medical Subject Headings
Adult; Aged; Cerebrospinal Fluid Rhinorrhea; Drainage; Female; Humans; Male; Middle Aged; Specimen Handling; Temperature; Time Factors; Transferrin