64482 Optimization of Acquisition Techniques and Image Analysis in Optical Polarization Imaging for Basal Cell Carcinoma Margin Detection Prior to Mohs Surgery
Recommended Citation
Abraskin K, Gaudette J, Mokhtari M, Jones B, Rosales Santillan M, Wuennenberg J, Lorizzo T, Yaroslavsky A, Ozog D, Kohli I. 64482 Optimization of Acquisition Techniques and Image Analysis in Optical Polarization Imaging for Basal Cell Carcinoma Margin Detection Prior to Mohs Surgery. J Am Acad Dermatol 2025; 93:AB260.
Document Type
Conference Proceeding
Publication Date
9-1-2025
Publication Title
J Am Acad Dermatol
Abstract
Background: Optical Polarization Imaging (OPI) exhibits potential for indirectly determining basal cell carcinoma (BCC) margins via detection of peri-lesional dermal collagen disruption. Although the capability of OPI in BCC margin assessment prior to Mohs surgery was recently published, challenges remain in image acquisition and analysis leading to discrepancies between OPI and histopathology findings. This pilot study was to identify key aspects for optimal OPI image acquisition and analyses. Methods: OPI images were collected from 27 BCC lesions enrolled in an IRB approved study. Images were reviewed for quality and comparisons were made between OPI and histopathology, after the first Mohs layer, to identify limitations and areas of improvement to increase consistency. Results: The following were identified to improve image quality: avoidance of bubbles within the gel by applying gentle pressure, selecting relatively flat areas as curvature was resulting in pressure gradient, application of uniform pressure, and using appropriate exposure time. To improve image analysis, the following were identified: utilization of skin marker with color transparent in the blue channel, avoidance of, or adjustment to image processing algorithm to account for excessive vascularization, photodamage, or hair in the field of view. Conclusion: OPI remains a promising modality for indirect detection of BCC margin and could potentially be added to other direct tumor imaging modalities for increased specificity. OPI is a user-friendly, non invasive and time efficient imaging technique; however, further refinement of image acquisition and analysis methods, incorporating the respective criteria above in future studies are warranted to optimize its capabilities.
Volume
93
First Page
AB260
