0650 Dysregulated innate MAIT cells in patients with hidradenitis suppurativa

Document Type

Conference Proceeding

Publication Date

8-1-2025

Publication Title

J Invest Dermatol

Abstract

Hidradenitis suppurativa (HS) is a chronic inflammatory skin condition characterized by painful and debilitating lesions. Limited studies have examined immune dysregulation in the peripheral blood and skin lesions of HS patients. Mucosal-associated invariant T (MAIT) cells, an innate T-cell subset responsive to bacterial threats, are implicated in autoimmune diseases, cancers, and inflammatory skin disorders. This study investigates the frequency and function of MAIT cells in HS patient blood and lesions. MAIT cells enriched from peripheral blood mononuclear cells (PBMCs) were isolated from HS patients and healthy controls for sc-RNA-Seq analysis. PBMCs and single cells from HS lesions were stained for surface markers (Vα7.2, CD3, CD161, CD4, CD8, MR1 tetramers) and intracellular cytokines (IL-17, TNFα, IFNgama) after stimulation. Flow cytometry analysis showed no change in overall MAIT cell frequency but revealed a significant reduction in IFNgama+ and TNFα+ MAIT cells in peripheral blood of HS patients. Subset analysis identified an increased frequency of CD4+ MAIT cells and a decreased frequency of CD4− MAIT cells in the periphery, with reduced CD4− IFNgama+ and TNFα+ MAIT cells. Additionally, HS lesions showed increased CD4+ MAIT cells and elevated CCL20 and CCL22 expression. These findings suggest that peripheral MAIT cells in HS patients are dysregulated, potentially migrating to skin lesions and contributing to disease pathogenesis.

Volume

145

Issue

8

First Page

S113

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