Head and neck tumor organoid grown under simplified media conditions model tumor biology and chemoradiation responses

Document Type

Article

Publication Date

7-7-2025

Publication Title

Sci Rep

Abstract

Head and neck squamous cell carcinoma (HNSCC) is a prevalent and often fatal malignancy associated with significant treatment-related toxicity. There is an urgent need for a preclinical model to assess therapeutic options and guide clinical decision-making. To define conditions for establishing patient-derived organoid (PDO) models that faithfully recapitulate morphological, histopathological, and genomic characteristics of HNSCC patients and can predict radiation and chemotherapy responses in patients, PDOs were generated from a group of HNSCC patients. The morphological, histological, mutational, and biological characteristics and treatment responses were evaluated. We demonstrate that the PDOs closely resemble resected tumors from which they were derived with respect to histopathology, differentiation state markers, p16 status, and mutation profiling. We observe patient-to-patient variation in cell proliferation rates. Additionally, they exhibit differential responses to radiotherapy and chemotherapy, which were examined using a cell viability assay. This methodology offers potential for drug screening in a pre-clinical context with the potential to mirror clinical outcomes. Our WNT-free growth conditions maintained the differentiation status of PDOs and enabled rapid assessment of drug response and the development of new models to identify new treatment options for head and neck cancer patients.

Medical Subject Headings

Humans; Organoids; Head and Neck Neoplasms; Chemoradiotherapy; Squamous Cell Carcinoma of Head and Neck; Cell Proliferation; Male; Cell Survival; Mutation; Female

PubMed ID

40624315

Volume

15

Issue

1

First Page

24221

Last Page

24221

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