Vascular heterogeneity influences treatment response in pancreatic cancer

Document Type

Conference Proceeding

Publication Date

9-28-2025

Abstract

Pancreatic ductal adenocarcinoma (PDAC) remains a highly lethal malignancy, in part due to its heterogeneous tumor microenvironment. Most PDAC tumors are poorly vascularized and embedded within densely fibrotic stroma; however, in prior computational analyses, we identified a subset of PDAC tumors with hyper-vascular features. In this study, we sought to determine the extent of vascular heterogeneity in PDAC and its functional consequences. Analysis of human tumor specimens revealed that approximately 10-20% of human tumors display features of hyper-vascularity. Using a panel of clonal murine cell lines that recapitulate this heterogeneity, we demonstrate that VEGFA-mediated angiogenesis does not account for the increased vessel density. Functionally, we further show that hyper-vascular tumors display enhanced sensitivity to both chemotherapy and anti-angiogenic therapy. These findings help explain the limited efficacy of VEGFA-targeted therapies in unselected PDAC cohorts and highlight vessel density as a potential biomarker for patient stratification.

Issue

18

First Page

A127

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