An antibody chemically and immunologically active against bovine testicular hyaluronidase was produced in rabbits by injecting them with purified hyaluronidase in complete Freund's adjuvant. Nonspecific antibodies, reacting with human and bovine proteins which had little or no hyaluronidase activity, were also detected by the double agar immunodiffusion technique using antiserum against these proteins. After absorption of the antiserum with bovine heart and liver and removal of the precipitation bands against human and bovine tissue proteins, the antiserum inhibited the chemical activity of the purified hyaluronidase and exhibited immunologic activity against the purified hyaluronidase as shown by the agar double immunodiffusion technique.
Fine, Gerald; Saeed, Sheikh M.; and LoGrippo, Gerald A.
"Bovine Testicular Antihyaluronidase in Rabbits: Production and Purification,"
Henry Ford Hospital Medical Journal
: Vol. 17
Available at: https://scholarlycommons.henryford.com/hfhmedjournal/vol17/iss1/4