Kidneys were preserved by continuous pulsatile perfusion and their metabolic rate monitored by O2 consumption. Experiments were carried out to determine if dialyzing the perfusate to supply metabolic substrates would aid in kidney preservation. A control group was compared against two experimental groups at 25°C. Average preservation time before fall off of oxygen consumption differed for each group: control - 8 hours, dialysis with amino acids - 6 hours, dialysis with amino acids and cofactors - 11 hours. Results indicated that, if suitable concentrations of metabolic substrates and cofactors were used, the preserved kidneys maintained higher levels of O2 consumption for longer periods of time.
Dall'Olmo, Daniel and Dienst, Stanley G.
"Prolonging oxygen consumption during preservation of canine kidneys by the addition of continuous dialysis,"
Henry Ford Hospital Medical Journal
: Vol. 24
Available at: https://scholarlycommons.henryford.com/hfhmedjournal/vol24/iss3/2