Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant

Authors

Somrita Dey
Smitha Gudipati, Henry Ford HealthFollow
Christopher Giuliano
Marcus J. Zervos, Henry Ford HealthFollow
Jonathan M. Monk
Richard Szubin
Sarah CJ Jorgensen
George Sakoulas
Andrew D. Berti

Recommended Citation

Dey S, Gudipati S, Giuliano C, Zervos MJ, Monk JM, Szubin R, Jorgensen SCJ, Sakoulas G, and Berti AD. Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia. Antibiotics (Basel) 2019; 9(1).

Document Type

Article

Publication Date

12-18-2019

Publication Title

Antibiotics (Basel)

Abstract

Immune biomarkers can stratify mortality risk in staphylococcal bacteremia. Microbial biomarkers may provide more consistent signals during early infection. We demonstrate that in ST45/USA600 bacteremia, bacterial membrane vesicle production in vitro predicts clinical mortality (773 vs. 116 RFU, survivors vs. decedents, p < 0.0001). Using a threshold of 301 relative fluorescence units (RFU), the sensitivity and specificity of the membrane vesicles to predict mortality are 78% and 90%, respectively. This platform is facile, scalable and can be integrated into clinical microbiology lab workflows.

PubMed ID

31861446

Volume

9

Issue

1