pH-Dependent Cellular Internalization of Paramagnetic Nanoparticle

Authors

Brana Janic, Henry Ford HealthFollow
Mohammed P. Bhuiyan, Henry Ford Health
James R. Ewing, Henry Ford HealthFollow
Meser M. Ali, Henry Ford HealthFollow

Recommended Citation

Janic B, Bhuiyan MP, Ewing JR, and Ali MM. pH-Dependent cellular internalization of paramagnetic nanoparticle. ACS Sens 2016; 1(8):975-978.

Document Type

Article

Publication Date

8-26-2016

Publication Title

ACS Sens

Abstract

A hallmark of the tumor microenvironment in malignant tumor is extracellular acidosis, which can be exploited for targeted delivery of drugs and imaging agents. A pH sensitive paramagnetic nanoaparticle (NP) is developed by incorporating GdDOTA-4AmP MRI contrast agent and pHLIP (pH Low Insertion Peptide) into the surface of a G5-PAMAM dendrimer. pHLIP showed pH-selective insertion and folding into cell membranes, but only in acidic conditions. We demonstrated that pHLIP-conjugated Gd44-G5 paramagnetic nanoparticle binds and fuses with cellular membrane at low pH, but not at normal physiological pH, and that it promotes cellular uptake. Intracellular trafficking of NPs showed endosomal/lysosomal path ways.

PubMed ID

28066811

Volume

1

Issue

8

First Page

975

Last Page

978