Recommended Citation
Wang L, Lu X, Chopp M, Li C, Zhang Y, Szalad A, Liu XS, and Zhang ZG. Comparative proteomic analysis of exosomes derived from endothelial cells and Schwann cells. PLoS One 2023; 18(8):e0290155.
Document Type
Article
Publication Date
8-18-2023
Publication Title
PLoS One
Abstract
Exosomes derived from endothelial cells and Schwann cells have been employed as novel treatments of neurological diseases, including peripheral neuropathy. Exosomal cargo plays a critical role in mediating recipient cell function. In this study, we thus performed a comprehensive proteomic analysis of exosomes derived from healthy mouse dermal microvascular endothelial cells (EC-Exo) and healthy mouse Schwann cells (SC-Exo). We detected 1,817and 1,579 proteins in EC-Exo and SC-Exo, respectively. Among them, 1506 proteins were present in both EC-Exo and SC-Exo, while 311 and 73 proteins were detected only in EC-Exo and SC-Exo, respectively. Bioinformatic analysis revealed that EC-Exo enriched proteins were involved in neurovascular function, while SC-Exo enriched proteins were related to lipid metabolism. Western blot analysis of 14 enriched proteins revealed that EC-Exo contained proteins involved in mediating endothelial function such as delta-like 4 (DLL4) and endothelial NOS (NOS3), whereas SC-Exo had proteins involved in mediating glial function such as apolipoprotein A-I (APOA1) and phospholipid transfer protein (PLTP). Collectively, the present study identifies differences in the cargo protein profiles of EC-Exo and SC-Exo, thus providing new molecular insights into their biological functions for the treatment of peripheral neuropathy.
Medical Subject Headings
Animals; Mice; Endothelial Cells; Exosomes; Proteomics; Schwann Cells; Neuroglia
PubMed ID
37594969
Volume
18
Issue
8
First Page
0290155
Last Page
0290155
