Nanoparticle Delivery of an Oligonucleotide Payload in a Glioblastoma Multiforme Animal Model
Recommended Citation
Kim BD, Mondal SK, Kenyon E, Chen M, Mallett CL, deCarvalho AC, Medarova Z, and Moore A. Nanoparticle Delivery of an Oligonucleotide Payload in a Glioblastoma Multiforme Animal Model. J Vis Exp 2024.
Document Type
Article
Publication Date
9-27-2024
Publication Title
J Vis Exp
Abstract
Glioblastoma multiforme (GBM) is the most common and aggressive form of primary brain malignancy for which there is no cure. The blood-brain barrier is a significant hurdle in the delivery of therapies to GBM. Reported here is an image-guided, iron oxide-based therapeutic delivery nano platform capable of bypassing this physiological barrier by virtue of size and accumulating in the tumor region, delivering its payload. This 25 nm nano platform consists of crosslinked dextran-coated iron oxide nanoparticles labeled with Cy5.5 fluorescent dye and containing antisense oligonucleotide as a payload. The magnetic iron oxide core enables tracking of the nanoparticles through in vivo magnetic resonance imaging, while Cy5.5 dye allows tracking by optical imaging. This report details the monitoring of the accumulation of this nanoparticle platform (termed MN-anti-miR10b) in orthotopically implanted glioblastoma tumors following intravenous injection. In addition, it provides insight into the in vivo delivery of RNA oligonucleotides, a problem that has hampered the translation of RNA therapeutics into the clinic.
Medical Subject Headings
Glioblastoma; Animals; Carbocyanines; Brain Neoplasms; Mice; Humans; MicroRNAs; Magnetic Iron Oxide Nanoparticles; Oligonucleotides, Antisense; Magnetic Resonance Imaging; Dextrans; Disease Models, Animal
PubMed ID
39400152
ePublication
ePub ahead of print
Issue
211
