Whole genome DNA is distinctly methylated in functioning and non-functioning pituitary macroadenomas
Mosella MS, Sabedot TS, Malta TM, Rock J, Felicella MM, Aho T, Poisson L, De Carvalho A, Noushmehr H, and Castro AVB. Whole genome DNA is distinctly methylated in functioning and non-functioning pituitary macroadenomas. Endocrine Reviews 2018; 39(2).
Few somatic mutations or epigenetic alterations were reported to be associated with pituitary adenomas (PA) functional status. In order to evaluate the role of global DNA methylation pattern in Functioning (FPA) and non-functioning PA (NFPA), we profiled the DNA methylome of 14 formalin- fixed paraffin-embedded pituitary specimens (5 non-tumoral, 9 macroadenomas, 6 NFPA; 5 FPA; 5 invasive; 3 large PA, 25±10 mm size) using the Human Illumina EPIC array platform (HM850K). Non-parametric supervised analyses of the methylomes were performed among NFPAs, FPAs and non-tumoral pituitary specimens; differentially methylated probes (DMPs)(p