Early-life wheeze trajectories are associated with distinct asthma transcriptomes later in life

Document Type

Article

Publication Date

4-4-2025

Publication Title

The Journal of allergy and clinical immunology

Abstract

BACKGROUND: Early childhood wheeze is characterized by heterogeneous trajectories having differential associations with later-life asthma development.

OBJECTIVE: We sought to determine how early-life wheeze trajectories impact later life asthma gene expression.

METHODS: The Children's Respiratory Environmental Workgroup is a collective of 12 birth cohorts, 7 of which conducted an additional visit with a nasal lavage collected and subjected to bulk RNA-sequencing. Early-life wheeze trajectories were defined using latent class analysis of longitudinal early-life wheezing data. Weighted gene correlation network analysis was used to associate gene expression patterns and current asthma with early-life wheeze trajectories.

RESULTS: We investigated 743 children (mean age, 17 ± 5.1 years; 360 [48.5%] male). Four patterns of early-life wheeze were identified: infrequent, transient, late-onset, and persistent. Early-life transient wheeze was associated with gene expression patterns related to increased antiviral response, and late-onset wheeze was associated with decreased insulin signaling and glucose metabolism. Early-life persistent wheeze was associated with gene expression modules of type 2 inflammation and epithelial development, but these modules did not distinguish those with current asthma. Children who had persistent wheeze in early life and current asthma displayed a unique increase in expression of genes enriched for neuronal processes and ciliated epithelial function compared with those without asthma.

CONCLUSIONS: Early-life longitudinal wheeze trajectories are associated with specific asthma transcriptomes later in life. These data suggest that early-life asthma prevention strategies may be most beneficial when tailored to the specific wheeze pattern.

Medical Subject Headings

Humans; Asthma/genetics/immunology; Respiratory Sounds/genetics/immunology; Male; Female; Transcriptome; Adolescent; Child; Child; Preschool; Longitudinal Studies; Wheeze; asthma; pediatrics; transcriptomics

PubMed ID

40189159

ePublication

ePub ahead of print

Volume

156

Issue

3

First Page

640

Last Page

650

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