Constant antioxidant supplementation increases blastocyst formation from oocyte donors

Document Type

Conference Proceeding

Publication Date

6-28-2023

Publication Title

Hum Reprod

Abstract

Study question: Does constant supplementation of antioxidants to the culture media increase blastocyst formation from oocyte donors? Summary answer: The constant supplementation of antioxidants to the culture media increases blastocyst formation from oocyte donors What is known already: The use of a combination of various antioxidants in embryo culture media has recently been investigated to explore the potential benefit for embryo development and clinical outcomes. However, the concentration of antioxidants added to the culture has only been determined by its effect. In this study, we performed a repeated antioxidants supplementation to resemble a physiological oxidation reduction environment. Therefore, we investigated the effect of antioxidants added every 12 hours to the culture media on blastocyst formation and expansion in oocyte donors. Study design, size, duration: This prospective study was conducted at CITMER, Mexico from April 2020 to November 2022.We included a total of 258 recipients from oocyte donors undergoing IVF/ICSI. Participants/materials, setting, methods: A total of 2403 zygotes were divided into 4 groups and cultured in the following conditions until blastocyst stage: Group 1A: 563 zygotes 20% O2 with antioxidants every 12 hours, Group 1B: 1109 zygotes 20% O2 with antioxidants at the beginning, Group 2A: 339 zygotes 5% O2 with antioxidants every 12 hours, Group 2B: 392 zygotes 5% O2 with antioxidants at the beginning. Embryo development was assessed. Odds ratio and Fisher test were performed. p<0.05=significant Main results and the role of chance: For both oxygen tensions, we found a significant increase in the total blastocyst formation rate (day 5+day 6) when antioxidants were added repeatedly (1A: 54.7% vs 1B: 45.9%, p=0.0007∗; 2A: 58.7% vs 2A: 46.9%, p=0.001∗). In addition, the rate of expansion at days 5 + 6 was also significantly higher than in the groups where the antioxidants were added only at the beginning of the culture (1A: 35.9% vs 1B: 29.9%, p=0.01∗; 2A: 41.3% vs 2A: 31.6%, p=0.006∗). Limitations, reasons for caution: Given this is a sibling zygotes study, patients are their own controls. Wider implications of the findings: Constant supplementation of antioxidants to the culture media increases blastocyst formation from oocyte donors as well as the expansion rate, which may significantly improve clinical outcomes.

Volume

38

First Page

i143

Last Page

i143

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