Suppression of FIP200 and autophagy by tumor-derived lactate promotes naïve T cell apoptosis and affects tumor immunity

Authors

Houjun Xia
Wei Wang
Joel Crespo
Ilona Kryczek
Wei Li
Shuang Wei
Zhaoqun Bian
Tomasz Maj
Mingxiao He
Rebecca J. Liu
Youwen He
Ramandeep Rattan, Henry Ford HealthFollow
Adnan R. Munkarah, Henry Ford HealthFollow
Jun-Lin Guan
Weiping Zou

Recommended Citation

Xia H, Wang W, Crespo J, Kryczek I, Li W, Wei S, Bian Z, Maj T, He M, Liu RJ, He Y, Rattan R, Munkarah A, Guan JL, and Zou W. Suppression of FIP200 and autophagy by tumor-derived lactate promotes naive T cell apoptosis and affects tumor immunity. Sci Immunol 2017; 2(17)

Document Type

Article

Publication Date

11-17-2017

Publication Title

Sci Immunol

Keywords

Animals, Apoptosis, Autophagy, Cell Line, Tumor, Female, Gene Expression Regulation, Neoplastic, Humans, Intracellular Signaling Peptides and Proteins, Lactic Acid, Male, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Neoplasms, Experimental, Ovarian Neoplasms, Protein-Tyrosine Kinases, T-Lymphocytes

Abstract

Naïve T cells are poorly studied in cancer patients. We report that naïve T cells are prone to undergo apoptosis due to a selective loss of FAK family-interacting protein of 200 kDa (FIP200) in ovarian cancer patients and tumor-bearing mice. This results in poor antitumor immunity via autophagy deficiency, mitochondria overactivation, and high reactive oxygen species production in T cells. Mechanistically, loss of FIP200 disables the balance between proapoptotic and antiapoptotic Bcl-2 family members via enhanced argonaute 2 (Ago2) degradation, reduced Ago2 and microRNA1198-5p complex formation, less microRNA1198-5p maturation, and consequently abolished microRNA1198-5p-mediated repression on apoptotic gene Bak1 Bcl-2 overexpression and mitochondria complex I inhibition rescue T cell apoptosis and promoted tumor immunity. Tumor-derived lactate translationally inhibits FIP200 expression by down-regulating the nicotinamide adenine dinucleotide level while potentially up-regulating the inhibitory effect of adenylate-uridylate-rich elements within the 3' untranslated region of Fip200 mRNA. Thus, tumors metabolically target naïve T cells to evade immunity.

Medical Subject Headings

Animals; Apoptosis; Autophagy; Cell Line; Tumor; Female; Gene Expression Regulation; Neoplastic; Humans; Intracellular Signaling Peptides and Proteins; Lactic Acid; Male; Mice; Inbred C57BL; Mice; Knockout; Mice; Transgenic; Neoplasms; Experimental; Ovarian Neoplasms; Protein-Tyrosine Kinases; T-Lymphocytes

PubMed ID

29150439

Volume

2

Issue

17